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Anti-Mitofusin 2 Antibody

144-62474
RayBiotech
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMFN2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Mitofusin 2 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID9927
  • Target name
    MFN2
  • Target description
    mitofusin 2
  • Target synonyms
    CMT2A, CMT2A2, CMT2A2A, CMT2A2B, CPRP1, HMSN6A, HSG, MARF, MSL, mitofusin-2, hyperplasia suppressor, mitochondrial assembly regulatory factor, transmembrane GTPase MFN2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95140
  • Protein Name
    Mitofusin-2
  • Scientific Description
    Mitofusin 2 pAb
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MFN2 antibody [N1N2], N-term (GTX102055) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
MFN2 antibody [N1N2], N-termGTX102055
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Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Muscle tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with Mitofusin 2 Polyclonal Antibody, Unconjugated (bs-2988R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
Mitofusin 2 Polyclonal AntibodyBS-2988R
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ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat
TargetMFN2
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Anti-Mitofusin 2 AntibodyA91370
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ReactivityHuman, Mouse, Rat
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Western Blot analysis of HuvEc cells using Mfn2 Polyclonal Antibody
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Mitofusin-2 Antibody(Rabbit)HY-P80752
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Figure 1. Western blot analysis of Mitofusin-2 using anti-Mitofusin-2 antibody (PB9265). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human Mitofusin-2 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin-2 antigen affinity purified polyclonal antibody (Catalog # PB9265) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin-2 at approximately 45KD. The expected band size for Mitofusin-2 is at 45KD.
Product group Antibodies
Boster Bio
Anti-Mitofusin 2/MFN2 Antibody Picoband(r)PB9265-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMFN2
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