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Western Blot analysis of HuvEc cells using Mfn2 Polyclonal Antibody
Western Blot analysis of HuvEc cells using Mfn2 Polyclonal Antibody
Western Blot analysis of HuvEc cells using Mfn2 Polyclonal Antibody

MFN2 Antibody

CSB-PA003234
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMFN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    MFN2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9927
  • Target name
    MFN2
  • Target description
    mitofusin 2
  • Target synonyms
    CMT2A; CMT2A2; CMT2A2A; CMT2A2B; CPRP1; HMSN6A; HSG; hyperplasia suppressor; MARF; mitochondrial assembly regulatory factor; mitofusin-2; transmembrane GTPase MFN2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95140
  • Protein Name
    Mitofusin-2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Muscle tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with Mitofusin 2 Polyclonal Antibody, Unconjugated (bs-2988R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
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Product group Antibodies
LifeSpan BioSciences
Mitofusin 2 / MFN2 Antibody (Internal)LS-C358807
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Figure 1. Western blot analysis of Mitofusin-2 using anti-Mitofusin-2 antibody (PB9265). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human Mitofusin-2 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin-2 antigen affinity purified polyclonal antibody (Catalog # PB9265) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin-2 at approximately 45KD. The expected band size for Mitofusin-2 is at 45KD.
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Anti-Mitofusin 2/MFN2 Antibody Picoband(r)PB9265-CARRIER-FREE
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