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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Anti-MLX Antibody

HPA052766
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMLX
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MLX
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6945
  • Target name
    MLX
  • Target description
    MAX dimerization protein MLX
  • Target synonyms
    MAD7, MXD7, TCFL4, TF4, bHLHd13, max-like protein X, BigMax protein, MAX-like bHLHZIP protein, MLX, MAX dimerization protein, class D basic helix-loop-helix protein 13, transcription factor-like protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UH92
  • Protein Name
    Max-like protein X
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MLX using anti-MLX antibody (A03087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLX antigen affinity purified polyclonal antibody (Catalog # A03087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLX at approximately 50 kDa. The expected band size for MLX is at 50 kDa.
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