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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA014643LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA014643LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA014643LA01HU at dilution of 1:100

MLX Antibody

CSB-PA014643LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMLX
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Overview

  • Supplier
    Cusabio
  • Product Name
    MLX Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6945
  • Target name
    MLX
  • Target description
    MAX dimerization protein MLX
  • Target synonyms
    bHLHd13; BigMax protein; class D basic helix-loop-helix protein 13; MAD7; MAX-like bHLHZIP protein; max-like protein X; MLX, MAX dimerization protein; MXD7; TCFL4; TF4; transcription factor-like protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UH92
  • Protein Name
    Max-like protein X
  • Scientific Description
    Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5-CACGTG-3. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MLX using anti-MLX antibody (A03087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLX antigen affinity purified polyclonal antibody (Catalog # A03087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLX at approximately 50 kDa. The expected band size for MLX is at 50 kDa.
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