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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Anti-MMS19 Antibody

HPA051936
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMMS19
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MMS19 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64210
  • Target name
    MMS19
  • Target description
    MMS19 cytosolic iron-sulfur assembly component
  • Target synonyms
    CIAO4, MET18, MMS19L, hMMS19, MMS19 nucleotide excision repair protein homolog, MET18 homolog, MMS19 homolog, cytosolic iron-sulfur assembly component, MMS19 nucleotide excision repair homolog, MMS19-like (MET18 homolog, S. cerevisiae), MMS19-like protein, homolog of yeast MMS19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96T76
  • Protein Name
    MMS19 nucleotide excision repair protein homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of MMS19 using anti-MMS19 antibody (A06218-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMS19 antigen affinity purified polyclonal antibody (Catalog # A06218-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMS19 at approximately 113 kDa. The expected band size for MMS19 is at 113 kDa.
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