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MMS19 Antibody

LS-C834249
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetMMS19
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MMS19 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.26 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID64210
  • Target name
    MMS19
  • Target description
    MMS19 cytosolic iron-sulfur assembly component
  • Target synonyms
    CIAO4, MET18, MMS19L, hMMS19, MMS19 nucleotide excision repair protein homolog, MET18 homolog, MMS19 homolog, cytosolic iron-sulfur assembly component, MMS19 nucleotide excision repair homolog, MMS19-like (MET18 homolog, S. cerevisiae), MMS19-like protein, homolog of yeast MMS19
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMS19 using anti-MMS19 antibody (A06218-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMS19 antigen affinity purified polyclonal antibody (Catalog # A06218-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMS19 at approximately 113 kDa. The expected band size for MMS19 is at 113 kDa.
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