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Anti-mouse IL-10 mAb (2A5), azide free

Anti-mouse IL-10 mAb (2A5), azide free

3431-5N
Mabtech
ApplicationsNeutralisation/Blocking
Product group Antibodies
TargetIl10
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-mouse IL-10 mAb (2A5), azide free
  • Delivery Days Customer
    9
  • Applications
    Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A5
  • Conjugate
    Unconjugated
  • Gene ID16153
  • Target name
    Il10
  • Target description
    interleukin 10
  • Target synonyms
    CSIF, If2a, Il-10, interleukin-10, cytokine synthesis inhibitory factor, interferon 2a
  • Host
    Rat
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables neutralization of mouse IL-10 bioactivity.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (A00021). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
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