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Anti-mouse IL-12/-23 (p40) mAb (C15.6), unconjugated

Anti-mouse IL-12/-23 (p40) mAb (C15.6), unconjugated

3451-3
Mabtech
ApplicationsELISA, ELISpot Assay
DownloadMSDS
Product group Antibodies
TargetIl12b
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-mouse IL-12/-23 (p40) mAb (C15.6), unconjugated
  • Delivery Days Customer
    9
  • Applications
    ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C15.6
  • Conjugate
    Unconjugated
  • Gene ID16160
  • Target name
    Il12b
  • Target description
    interleukin 12b
  • Target synonyms
    CLMF p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; Il-1; Il-12; IL-12 p40 subunit; IL-12 subunit p40; Il-12b; Il12p40; Il-12p40; IL-23 subunit p40; interleukin-12 p40 subunit; interleukin-12 subunit beta; p40
  • Host
    Rat
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of the p40 subunit of mouse IL-12 and IL-23 in immunoassays such as ELISpot and ELISA.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL12B at approximately 37-45KD. The expected band size for IL12B is at 37KD.
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