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Goat anti-Il12b / Il12p40 (mouse)

EB07863
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIl12b
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Il12b / Il12p40 (mouse) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID16160
  • Target name
    Il12b
  • Target description
    interleukin 12b
  • Target synonyms
    CLMF p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; Il-1; Il-12; IL-12 p40 subunit; IL-12 subunit p40; Il-12b; Il12p40; Il-12p40; IL-23 subunit p40; interleukin-12 p40 subunit; interleukin-12 subunit beta; p40
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_032378.1. GeneIDs all Nonhuman: 16160 (mouse); 64546 (rat);. Purification: Antigen affinity purified. Names and symbols: IL12B; Il12p40; interleukin 12B; RP23-388G23.1; Il-12b; Il-12p40; p40; IL-12 p40; IL-23 subunit p40
  • Reactivity Supplier
    Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Effects of acupuncture on LIF and IL-12 in rats of implantation failure. Gui J et al., 2012 May, Am J Reprod Immunol
    Read more

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Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL12B at approximately 37-45KD. The expected band size for IL12B is at 37KD.
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