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Anti-Mouse Il12b (Center) Antibody

102-23305
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetIl12b
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Mouse Il12b (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB38646
  • Gene ID16160
  • Target name
    Il12b
  • Target description
    interleukin 12b
  • Target synonyms
    Il-12b, Il-12p40, Il12p40, p40, interleukin-12 subunit beta, CLMF p40, IL-12 p40 subunit, IL-12 subunit p40, IL-23 subunit p40, cytotoxic lymphocyte maturation factor 40 kDa subunit, interleukin-12 p40 subunit
  • Host
    Rabbit
  • Protein IDP43432
  • Protein Name
    Interleukin-12 subunit beta
  • Scientific Description
    Rabbit Anti-Mouse Il12b (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL12B at approximately 37-45KD. The expected band size for IL12B is at 37KD.
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