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Anti-mouse IL-6 mAb (32C11), biotin

Anti-mouse IL-6 mAb (32C11), biotin

3361-6
Mabtech
ApplicationsELISA, ELISpot Assay
Product group Antibodies
TargetIl6
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-mouse IL-6 mAb (32C11), biotin
  • Delivery Days Customer
    9
  • Applications
    ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    32C11
  • Conjugate
    Biotin
  • Gene ID16193
  • Target name
    Il6
  • Target description
    interleukin 6
  • Target synonyms
    Il-6, interleukin-6, B-cell hybridoma growth factor, interleukin HP-1
  • Host
    Rat
  • Isotype
    IgG2a
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of mouse IL-6 in immunoassays such as ELISpot, FluoroSpot, and ELISA.
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of IL6 using anti-IL6 antibody (PB9034). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse J774A.1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates, Lane 3: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # PB9034) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
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