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Anti-Mouse THEMIS (C-term) Antibody

102-20777
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTHEMIS
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Mouse THEMIS (C-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB28989
  • Gene ID387357
  • Target name
    THEMIS
  • Target description
    thymocyte selection associated
  • Target synonyms
    C6orf190, C6orf207, GASP, SPOT, THEMIS1, TSEPA, protein THEMIS, GRB2-associated protein, signaling phosphoprotein specific for T cells, thymocyte selection pathway associated, thymocyte-expressed molecule involved in selection
  • Host
    Rabbit
  • Protein IDQ8N1K5
  • Protein Name
    Protein THEMIS
  • Scientific Description
    Rabbit Anti-THEMIS (C-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of THEMIS using anti-THEMIS antibody (A07854-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THEMIS antigen affinity purified polyclonal antibody (Catalog # A07854-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THEMIS at approximately 73 kDa. The expected band size for THEMIS is at 73 kDa.
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