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Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-MSH2 rabbit monoclonal antibody (Clone RM375) at a 1:200 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-MSH2 rabbit monoclonal antibody (Clone RM375) at a 1:200 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-MSH2 rabbit monoclonal antibody (Clone RM375) at a 1:200 dilution.

anti-MSH2 (human), Rabbit Monoclonal (RM375)

REV-31-1261-00
RevMAb Biosciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMSH2
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Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-MSH2 (human), Rabbit Monoclonal (RM375)
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM375
  • Gene ID4436
  • Target name
    MSH2
  • Target description
    mutS homolog 2
  • Target synonyms
    COCA1; DNA mismatch repair protein Msh2; DNA mismatch repair protein Msh2 transcript; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; MMRCS2; mutS homolog 2, colon cancer, nonpolyposis type 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43246
  • Protein Name
    DNA mismatch repair protein Msh2
  • Scientific Description
    Recombinant Antibody. This antibody reacts to human DNA mismatch repair protein Msh2. Applications: WB, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. The mismatch repair (MMR) proteins are required to maintain genomic integrity in prokaryotes and eukaryotes, by correcting single mismatches and short unpaired regions, such as small insertions and deletions. In eukaryotes, three proteins are involved in mismatch recognition, MSH2, MSH3 and MSH6. The three proteins form two heterodimers MutSalpha (MSH2-MSH6) and MutSbeta (MSH2-MSH3). MutSalpha is thought to be involved primarily in the recognition and repair of base-base mismatches and small insertion/deletion loops. MutSbeta acts preferentially on insertion/deletion loops up to 12 nucleotides in length. The MSH2, MSH3, and PMS2 mismatch repair proteins are also involved in other DNA repair pathways such as single-strand annealing and homologous recombination, anti-recombination, DNA damage signaling, apoptosis, as well as site-specific mutagenesis during immunoglobin somatic hypermutation and class switch recombination. They interact with several other oncogenic targets, including ATR, BRCA1 or p53. Deficiencies in expression of DNA repair genes underlie many forms of cancer. If DNA repair is deficient, DNA damage tends to accumulate. Such excess DNA damage may increase mutations due to error-prone translesion synthesis and error prone repair. Elevated DNA damage may also increase epigenetic alterations due to errors during DNA repair. Such mutations and epigenetic alterations may give rise to cancer. MSH2 mutation is a commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MSH2 mutations have also been linked to endometrial cancer and the development of endometrial carcinomas. - The mismatch repair (MMR) proteins are required to maintain genomic integrity in prokaryotes and eukaryotes, by correcting single mismatches and short unpaired regions, such as small insertions and deletions. In eukaryotes, three proteins are involved in mismatch recognition, MSH2, MSH3 and MSH6. The three proteins form two heterodimers MutSalpha (MSH2-MSH6) and MutSbeta (MSH2-MSH3). MutSalpha is thought to be involved primarily in the recognition and repair of base-base mismatches and small insertion/deletion loops. MutSbeta acts preferentially on insertion/deletion loops up to 12 nucleotides in length. The MSH2, MSH3, and PMS2 mismatch repair proteins are also involved in other DNA repair pathways such as single-strand annealing and homologous recombination, anti-recombination, DNA damage signaling, apoptosis, as well as site-specific mutagenesis during immunoglobin somatic hypermutation and class switch recombination. They interact with several other oncogenic targets, including ATR, BRCA1 or p53. Deficiencies in expression of DNA repair genes underlie many forms of cancer. If DNA repair is deficient, DNA damage tends to accumulate. Such excess DNA damage may increase mutations due to error-prone translesion synthesis and error prone repair. Elevated DNA damage may also increase epigenetic alterations due to errors during DNA repair. Such mutations and epigenetic alterations may give rise to cancer. MSH2 mutation is a commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MSH2 mutations have also been linked to endometrial cancer and the development of endometrial carcinomas.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human SW480 cell lysates probed with MSH2 Polyclonal Antibody, Unconjugated (bs-0758R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of MSH2 using anti-MSH2 antibody (A00140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody (Catalog # A00140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSH2 at approximately 105 kDa. The expected band size for MSH2 is at 105 kDa.
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