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Immunohistochemical staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.

Anti-MTDH Antibody

AMAB90762
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMTDH
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MTDH
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL0397
  • Conjugate
    Unconjugated
  • Gene ID92140
  • Target name
    MTDH
  • Target description
    metadherin
  • Target synonyms
    3D3, AEG-1, AEG1, LYRIC, LYRIC/3D3, protein LYRIC, 3D3/LYRIC, astrocyte elevated gene 1, astrocyte elevated gene-1 protein, lysine-rich CEACAM1 co-isolated protein, metastasis adhesion protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ86UE4
  • Protein Name
    Protein LYRIC
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of LYRIC using anti-LYRIC antibody (PB9338). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: U-87MG whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: MDA-MB-453 whole cell lysates, Lane 4: PC-3 whole cell lysates, Lane 5: A431 whole cell lysates, Lane 6: CACO-2 whole cell lysates, Lane 7: HepG2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYRIC antigen affinity purified polyclonal antibody (Catalog # PB9338) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYRIC at approximately 70-80KD. The expected band size for LYRIC is at 70-80KD.
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