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Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-AEG-1/LYRIC Polyclonal Antibody, Unconjugated (bs-2231R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-AEG-1/LYRIC Polyclonal Antibody, Unconjugated (bs-2231R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-AEG-1/LYRIC Polyclonal Antibody, Unconjugated (bs-2231R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

LYRIC Polyclonal Antibody

BS-2231R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMTDH
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    LYRIC Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID92140
  • Target name
    MTDH
  • Target description
    metadherin
  • Target synonyms
    3D3; 3D3/LYRIC; AEG1; AEG-1; astrocyte elevated gene 1; astrocyte elevated gene-1 protein; LYRIC; LYRIC/3D3; lysine-rich CEACAM1 co-isolated protein; metastasis adhesion protein; protein LYRIC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UE4
  • Protein Name
    Protein LYRIC
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Astrocyte elevated gene-1 overexpression in histologically favorable Wilms tumor is related to poor prognosis. Guo F et al., 2014 Apr, J Pediatr Urol
    Read more

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Figure 1. Western blot analysis of LYRIC using anti-LYRIC antibody (PB9338). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: U-87MG whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: MDA-MB-453 whole cell lysates, Lane 4: PC-3 whole cell lysates, Lane 5: A431 whole cell lysates, Lane 6: CACO-2 whole cell lysates, Lane 7: HepG2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYRIC antigen affinity purified polyclonal antibody (Catalog # PB9338) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYRIC at approximately 70-80KD. The expected band size for LYRIC is at 70-80KD.
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