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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA773028ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA773028ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA773028ESR2HU at dilution of 1:100

MTDH Antibody

CSB-PA773028ESR2HU
Cusabio
ApplicationsImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMTDH
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Overview

  • Supplier
    Cusabio
  • Product Name
    MTDH Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92140
  • Target name
    MTDH
  • Target description
    metadherin
  • Target synonyms
    3D3; 3D3/LYRIC; AEG1; AEG-1; astrocyte elevated gene 1; astrocyte elevated gene-1 protein; LYRIC; LYRIC/3D3; lysine-rich CEACAM1 co-isolated protein; metastasis adhesion protein; protein LYRIC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UE4
  • Protein Name
    Protein LYRIC
  • Scientific Description
    Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LYRIC using anti-LYRIC antibody (PB9338). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: U-87MG whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: MDA-MB-453 whole cell lysates, Lane 4: PC-3 whole cell lysates, Lane 5: A431 whole cell lysates, Lane 6: CACO-2 whole cell lysates, Lane 7: HepG2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYRIC antigen affinity purified polyclonal antibody (Catalog # PB9338) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYRIC at approximately 70-80KD. The expected band size for LYRIC is at 70-80KD.
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