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Western blot analysis of lysates from HUVEC and HeLa cells, using MtSSB Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HUVEC and HeLa cells, using MtSSB Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HUVEC and HeLa cells, using MtSSB Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-MtSSB SSBP1 Antibody

A30475
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetSSBP1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-MtSSB SSBP1 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID6742
  • Target name
    SSBP1
  • Target description
    single stranded DNA binding protein 1
  • Target synonyms
    Mt-SSB, OPA13, SOSS-B1, SSBP, mtSSB, single-stranded DNA-binding protein, mitochondrial, PWP1-interacting protein 17, single-stranded DNA binding protein 1, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04837
  • Protein Name
    Single-stranded DNA-binding protein, mitochondrial
  • Scientific Description
    Boster Bio Anti-MtSSB SSBP1 Antibody (Catalog# A30475). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Cow.
  • Reactivity
    Bovine, Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SSBP1 using anti-SSBP1 ntibody (A05166-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSBP1 antigen affinity purified polyclonal antibody (Catalog # A05166-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSBP1 at approximately 16 kDa. The expected band size for SSBP1 is at 17-18 kDa.
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