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Immunohistochemical staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts.

Anti-MYBL1 Antibody

HPA008791
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetMYBL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MYBL1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4603
  • Target name
    MYBL1
  • Target description
    MYB proto-oncogene like 1
  • Target synonyms
    AMYB; A-MYB; myb-like protein 1; myb-related protein A; v-myb avian myeloblastosis viral oncogene homolog-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10243
  • Protein Name
    Myb-related protein A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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ICC/IF analysis of PFA-fixed A549 cells using GTX03441 A-Myb antibody. Permeabilization : 0.1% Triton X-100 Red : Primary antibody Green : beta tubulin Blue : DAPI
Product group Antibodies
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Figure 1. Western blot analysis of v-Myb/MYBL1 using anti-v-Myb/MYBL1 antibody (A05803-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-v-Myb/MYBL1 antigen affinity purified polyclonal antibody (Catalog # A05803-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for v-Myb/MYBL1 at approximately 86KD. The expected band size for v-Myb/MYBL1 is at 86KD.
Product group Antibodies
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Anti-v-Myb/MYBL1 Antibody Picoband(r)A05803-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetMYBL1
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