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ProductsBack/MYBL1 / A-MYB Antibody

MYBL1 / A-MYB Antibody

LS-C411352
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMYBL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MYBL1 / A-MYB Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 78kDa/85kDa, while the observed MW by Western blot was 110kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 78kDa/85kDa, while the observed MW by Western blot was 110kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4603
  • Target name
    MYBL1
  • Target description
    MYB proto-oncogene like 1
  • Target synonyms
    A-MYB, AMYB, myb-related protein A, myb-like protein 1, v-myb avian myeloblastosis viral oncogene homolog-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of v-Myb/MYBL1 using anti-v-Myb/MYBL1 antibody (A05803-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-v-Myb/MYBL1 antigen affinity purified polyclonal antibody (Catalog # A05803-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for v-Myb/MYBL1 at approximately 86KD. The expected band size for v-Myb/MYBL1 is at 86KD.
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ICC/IF analysis of PFA-fixed A549 cells using GTX03441 A-Myb antibody. Permeabilization : 0.1% Triton X-100 Red : Primary antibody Green : beta tubulin Blue : DAPI
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