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MYBL1 Antibody

CSB-PA010296
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMYBL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYBL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4603
  • Target name
    MYBL1
  • Target description
    MYB proto-oncogene like 1
  • Target synonyms
    A-MYB, AMYB, myb-related protein A, myb-like protein 1, v-myb avian myeloblastosis viral oncogene homolog-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10243
  • Protein Name
    Myb-related protein A
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of v-Myb/MYBL1 using anti-v-Myb/MYBL1 antibody (A05803-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-v-Myb/MYBL1 antigen affinity purified polyclonal antibody (Catalog # A05803-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for v-Myb/MYBL1 at approximately 86KD. The expected band size for v-Myb/MYBL1 is at 86KD.
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