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Anti-myc [8]

Ab02771-10.0
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMYC
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-myc [8]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is recommneded for the identification and analysis of myc via Western blot, immunoprecipitation and immunohistochemistry.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IP; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8
  • Gene ID4609
  • Target name
    MYC
  • Target description
    MYC proto-oncogene, bHLH transcription factor
  • Target synonyms
    avian myelocytomatosis viral oncogene homolog; bHLHe39; class E basic helix-loop-helix protein 39; c-Myc; MRTL; myc proto-oncogene protein; MYCC; myc-related translation/localization regulatory factor; proto-oncogene c-Myc; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP01106
  • Protein Name
    Myc proto-oncogene protein
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human, Mouse, Rat
  • Reactivity Supplier Note
    The hybridoma of the original format was produced by the fusion of splenocytes from mice immunized with recombinant full-length human c-Myc protein and mouse myeloma cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C-Myc/MYC using anti-C-Myc/MYC antibody (A00026-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Myc/MYC antigen affinity purified polyclonal antibody (Catalog # A00026-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Myc/MYC at approximately 65 kDa. The expected band size for C-Myc/MYC is at 65 kDa.
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