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Figure 1. Western blotting validation for Anti-Myotilin MYOT Antibody A04663 WesternBlot (WB) analysis of MYOT polyclonal antibody Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-Myotilin MYOT Antibody A04663 WesternBlot (WB) analysis of MYOT polyclonal antibody Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-Myotilin MYOT Antibody A04663 WesternBlot (WB) analysis of MYOT polyclonal antibody Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Anti-Myotilin MYOT Antibody

A04663
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMYOT
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Myotilin MYOT Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID9499
  • Target name
    MYOT
  • Target description
    myotilin
  • Target synonyms
    LGMD1, LGMD1A, MFM3, TTID, TTOD, myotilin, 57 kDa cytoskeletal protein, myofibrillar titin-like Ig domains protein, titin immunoglobulin domain protein (myotilin)
  • Host
    Rabbit
  • Protein IDQ9UBF9
  • Protein Name
    Myotilin
  • Scientific Description
    Boster Bio Anti-Myotilin MYOT Antibody catalog # A04663. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYOT using anti-MYOT antibody (A04663-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYOT antigen affinity purified polyclonal antibody (Catalog # A04663-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYOT at approximately 55 kDa. The expected band size for MYOT is at 55 kDa.
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Immunohistochemistry analysis in human skeletal muscle and skin tissues using Anti-MYOT antibody. Corresponding MYOT RNA-seq data are presented for the same tissues.
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