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Immunohistochemical staining of human esophagus shows nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows nuclear positivity in squamous epithelial cells.

Anti-NACC1 Antibody

HPA021238
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNACC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NACC1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID112939
  • Target name
    NACC1
  • Target description
    nucleus accumbens associated 1
  • Target synonyms
    BEND8, BTBD14B, BTBD30, NAC-1, NAC1, NECFM, nucleus accumbens-associated protein 1, BEN domain containing 8, BTB/POZ domain-containing protein 14B, nucleus accumbens associated 1, BEN and BTB (POZ) domain containing, transcriptional repressor NAC1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96RE7
  • Protein Name
    Nucleus accumbens-associated protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (A08675-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nac1/NACC1 antigen affinity purified polyclonal antibody (Catalog # A08675-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nac1/NACC1 at approximately 68 kDa. The expected band size for Nac1/NACC1 is at 57 kDa.
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