Western blot analysis using anti-Nampt (human), pAb (Prod. No. AG-25A-0025) at 1:2000 dilution. 1: Human Nampt (His-tagged). 2: LPS-treated human peripheral blood leukocyte lysate.
Western blot analysis using anti-Nampt (human), pAb (Prod. No. AG-25A-0025) at 1:2000 dilution. 1: Human Nampt (His-tagged). 2: LPS-treated human peripheral blood leukocyte lysate.
anti-Nampt (Visfatin/PBEF) (human), pAb
AG-25A-0025
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNAMPT
Overview
- SupplierAdipoGen Life Sciences
- Product Nameanti-Nampt (Visfatin/PBEF) (human), pAb
- Delivery Days Customer10
- Antibody SpecificityRecognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- Gene ID10135
- Target nameNAMPT
- Target descriptionnicotinamide phosphoribosyltransferase
- Target synonyms1110035O14Rik; NAmPRTase; nicotinamide phosphoribosyltransferase; PBEF; PBEF1; pre-B cell-enhancing factor; pre-B-cell colony-enhancing factor 1; VF; VISFATIN
- HostRabbit
- Protein IDP43490
- Protein NameNicotinamide phosphoribosyltransferase
- Scientific DescriptionNampt also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. - Polyclonal Antibody. Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot. Source: Rabbit. Applications: ELISA, IHC, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. Nampt also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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![Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10microl per 50microl reaction mix were applied in the reaction mix (20](https://adipogen.com/pub/media/catalog/product/a/g/ag-40a-0018_h-nampt_visfatin_-his-enzymatic-activity.png)
![Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10microl per 50microl reaction mix were applied in the reaction mix (20](https://adipogen.com/pub/media/catalog/product/a/g/ag-40a-0018aa_h-nampt_visfatin_-his-enzymatic-activity.png)