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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.

Anti-NARFL Antibody

HPA040851
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCIAO3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NARFL
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64428
  • Target name
    CIAO3
  • Target description
    cytosolic iron-sulfur assembly component 3
  • Target synonyms
    cytosolic Fe-S cluster assembly factor NARFL; cytosolic iron-sulfur assembly component 3; HPRN; IOP1; iron-only hydrogenase-like protein 1; LET1 like/JFP15; LET1L; NAR1; NARFL; nuclear prelamin A recognition factor like; PRN; protein related to Narf
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6Q4
  • Protein Name
    Cytosolic Fe-S cluster assembly factor NARFL
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NARFL/CIAO3 using anti-NARFL/CIAO3 antibody (A32387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARFL/CIAO3 antigen affinity purified polyclonal antibody (A32387) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARFL/CIAO3 at approximately 53 kDa. The expected band size for NARFL/CIAO3 is at 53 kDa.
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