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NARFL antibody

GTX116764
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCIAO3
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Overview

  • Supplier
    GeneTex
  • Product Name
    NARFL antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.72 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64428
  • Target name
    CIAO3
  • Target description
    cytosolic iron-sulfur assembly component 3
  • Target synonyms
    HPRN, IOP1, LET1L, NAR1, NARFL, PRN, cytosolic iron-sulfur assembly component 3, LET1 like/JFP15, cytosolic Fe-S cluster assembly factor NARFL, iron-only hydrogenase-like protein 1, nuclear prelamin A recognition factor like, protein related to Narf
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6Q4
  • Protein Name
    Cytosolic iron-sulfur assembly component 3
  • Scientific Description
    Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wu H, Wei H, Zhang D, et al. Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy. Redox Biol. 2020,36:101661. doi: 10.1016/j.redox.2020.101661
    Read this paper
  • Ast T, Meisel JD, Patra S, et al. Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis. Cell. 2019,177(6):1507-1521.e16. doi: 10.1016/j.cell.2019.03.045
    Read this paper

Datasheet

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Figure 1. Western blot analysis of NARFL/CIAO3 using anti-NARFL/CIAO3 antibody (A32387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARFL/CIAO3 antigen affinity purified polyclonal antibody (A32387) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARFL/CIAO3 at approximately 53 kDa. The expected band size for NARFL/CIAO3 is at 53 kDa.
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