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Western Blot analysis of A549 cells using NARFL Polyclonal Antibody
Western Blot analysis of A549 cells using NARFL Polyclonal Antibody
Western Blot analysis of A549 cells using NARFL Polyclonal Antibody

NARFL Antibody

CSB-PA003370
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCIAO3
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Overview

  • Supplier
    Cusabio
  • Product Name
    NARFL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64428
  • Target name
    CIAO3
  • Target description
    cytosolic iron-sulfur assembly component 3
  • Target synonyms
    HPRN, IOP1, LET1L, NAR1, NARFL, PRN, cytosolic iron-sulfur assembly component 3, LET1 like/JFP15, cytosolic Fe-S cluster assembly factor NARFL, iron-only hydrogenase-like protein 1, nuclear prelamin A recognition factor like, protein related to Narf
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6Q4
  • Protein Name
    Cytosolic Fe-S cluster assembly factor NARFL
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NARFL/CIAO3 using anti-NARFL/CIAO3 antibody (A32387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARFL/CIAO3 antigen affinity purified polyclonal antibody (A32387) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARFL/CIAO3 at approximately 53 kDa. The expected band size for NARFL/CIAO3 is at 53 kDa.
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