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Anti-NECAB1 Antibody

144-63957
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetNECAB1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-NECAB1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID64168
  • Target name
    NECAB1
  • Target description
    N-terminal EF-hand calcium binding protein 1
  • Target synonyms
    EFCBP1, STIP-1, N-terminal EF-hand calcium-binding protein 1, EF-hand calcium-binding protein 1, neuronal calcium binding protein, neuronal calcium-binding protein 1, synaptotagmin interacting protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N987
  • Protein Name
    N-terminal EF-hand calcium-binding protein 1
  • Scientific Description
    NECAB1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Immunofluorescence staining of U251 cells with CSB-PA843298LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB1 antigen affinity purified polyclonal antibody (Catalog # A13973-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NECAB1 at approximately 41 kDa. The expected band size for NECAB1 is at 41 kDa.
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Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using AMAb90798 antibody. Corresponding NECAB1 RNA-seq data are presented for the same tissues.
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