Figure 1. Western blot analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB1 antigen affinity purified polyclonal antibody (Catalog # A13973-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NECAB1 at approximately 41 kDa. The expected band size for NECAB1 is at 41 kDa.
Figure 1. Western blot analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB1 antigen affinity purified polyclonal antibody (Catalog # A13973-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NECAB1 at approximately 41 kDa. The expected band size for NECAB1 is at 41 kDa.
Anti-NECAB1 Antibody Picoband(r)
A13973-1-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetNECAB1
Overview
- SupplierBoster Bio
- Product NameAnti-NECAB1 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID64168
- Target nameNECAB1
- Target descriptionN-terminal EF-hand calcium binding protein 1
- Target synonymsEFCBP1; EF-hand calcium-binding protein 1; neuronal calcium binding protein; neuronal calcium-binding protein 1; N-terminal EF-hand calcium-binding protein 1; STIP-1; synaptotagmin interacting protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ8N987
- Protein NameN-terminal EF-hand calcium-binding protein 1
- Scientific DescriptionBoster Bio Anti-NECAB1 Antibody Picoband® catalog # A13973-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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