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Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.
Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.
Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.

Anti-NES Antibody

AMAB90556
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNES
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NES
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL0197
  • Conjugate
    Unconjugated
  • Gene ID10763
  • Target name
    NES
  • Target description
    nestin
  • Target synonyms
    Nbla00170; nestin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP48681
  • Protein Name
    Nestin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Nestin using anti-Nestin antibody (PB9874). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nestin antigen affinity purified polyclonal antibody (Catalog # PB9874) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nestin at approximately 230 kDa. The expected band size for Nestin is at 177 kDa.
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