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Mouse anti Nestin

MUB1302P
Nordic-MUbio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNES
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Nestin
  • Delivery Days Customer
    7
  • Application Supplier Note
    The 10C2 nestin antibody is useful for immunocytochemistry on fixed cells, immunohistochemistry on frozen and paraffin-embedded tissues, and Western blotting. Optimal antibody dilution should be determined by titration; recommended range is 1:100 to 1:200 for immunocytochemistry and immunohistochemistry with a fluorescein or rhodamine conjugated anti-mouse secondary antibody, and 1:1000 to 1:5000 for Western blot applications using chemoluminescent detection.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10C2
  • Gene ID10763
  • Target name
    NES
  • Target description
    nestin
  • Target synonyms
    Nbla00170; nestin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP48681
  • Protein Name
    Nestin
  • Source
    Monoclonal antibody 10C2 was derived by fusion of P3x63Ag8.653 myeloma cells with spleen cells from a BALB/c mouse immunized with a 150-amino-acid fragment from the cloned human nestin, conjugated to glutathione S-transferase.
  • Reactivity Supplier
    Human;macaque;Monkey
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nestin using anti-Nestin antibody (PB9874). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nestin antigen affinity purified polyclonal antibody (Catalog # PB9874) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nestin at approximately 230 kDa. The expected band size for Nestin is at 177 kDa.
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