More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Nestin Monoclonal Antibody

Nestin Monoclonal Antibody

BSM-60784M
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetNES
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    Nestin Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    E2G4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10763
  • Target name
    NES
  • Target description
    nestin
  • Target synonyms
    Nbla00170, nestin
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP48681
  • Protein Name
    Nestin
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Immunofluorescent analysis of U251 cells using CSB-PA015713YA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Product group Antibodies
Cusabio
NES AntibodyCSB-PA015713YA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetNES
  • SizePrice
Anti-Nestin Antibody
Product group Antibodies
Antibodies.com
Anti-Nestin AntibodyA104326
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NES Antibody130-10370
ApplicationsWestern Blot, ELISA
TargetNES
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.
Product group Antibodies
Atlas Antibodies
Anti-NES AntibodyAMAB90556
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetNES
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
NES / Nestin Antibody (clone 2C10-G10-G9, Preservative Free)LS-C743715
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetNES
  • SizePrice
Product group Antibodies
MedChem Express
Nestin Antibody(Rabbit)HY-P81120
TargetNES
  • SizePrice
Product group Antibodies
Nordic-MUbio
Mouse anti NestinMUB1302P
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Monkey, Primate
TargetNES
  • SizePrice
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody (GTX116066) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
Nestin antibodyGTX116066
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Rabbit
TargetNES
  • SizePrice
Figure 1. Western blot analysis of Nestin using anti-Nestin antibody (PB9874). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nestin antigen affinity purified polyclonal antibody (Catalog # PB9874) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nestin at approximately 230 kDa. The expected band size for Nestin is at 177 kDa.
Product group Antibodies
Boster Bio
Anti-Nestin Antibody Picoband(r)PB9874-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNES
  • SizePrice
Product group Antibodies
Biomatik
Anti-Nestin (NES) Monoclonal AntibodyCAU36302
ApplicationsFlow Cytometry
TargetNES
  • SizePrice