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Immunohistochemical staining of human cervix shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human cervix shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human cervix shows moderate nuclear positivity in squamous epithelial cells.

Anti-NFAT5 Antibody

HPA069711
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetNFAT5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NFAT5
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10725
  • Target name
    NFAT5
  • Target description
    nuclear factor of activated T cells 5
  • Target synonyms
    glutamine rich protein H65; NF-AT5; NFATL1; NFAT-like protein 1; NFATZ; nuclear factor of activated T-cells 5; nuclear factor of activated T-cells 5, tonicity-responsive; OREBP; osmotic response element-binding protein; T-cell transcription factor NFAT5; TonE-binding protein; TONEBP; tonicity-responsive enhancer-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94916
  • Protein Name
    Nuclear factor of activated T-cells 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NFAT5 using anti-NFAT5 antibody (A01815-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT5 antigen affinity purified polyclonal antibody (Catalog # A01815-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT5 at approximately 170KD. The expected band size for NFAT5 is at 170KD.
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