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NFAT5 Polyclonal Antibody

BS-9473R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNFAT5
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Overview

  • Supplier
    Bioss
  • Product Name
    NFAT5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), ELISA(ELISA=1:5000-10000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500), ICC/IF(ICC/IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID10725
  • Target name
    NFAT5
  • Target description
    nuclear factor of activated T cells 5
  • Target synonyms
    NF-AT5, NFATL1, NFATZ, OREBP, TONEBP, nuclear factor of activated T-cells 5, NFAT-like protein 1, T-cell transcription factor NFAT5, TonE-binding protein, glutamine rich protein H65, nuclear factor of activated T-cells 5, tonicity-responsive, osmotic response element-binding protein, tonicity-responsive enhancer-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • ASIC1a induces synovial inflammation via the Ca2+/NFATc3/ RANTES pathway. Zhang Y et al., 2020, Theranostics
    Read more
  • NFAT5 is Regulated by p53/miR-27a Signal Axis and Promotes Mouse Ovarian Granulosa Cells Proliferation. Tao H et al., 2019, Int J Biol Sci
    Read more

Datasheet

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Figure 1. Western blot analysis of NFAT5 using anti-NFAT5 antibody (A01815-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT5 antigen affinity purified polyclonal antibody (Catalog # A01815-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT5 at approximately 170KD. The expected band size for NFAT5 is at 170KD.
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