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Anti-NGFR Antibody

144-02097
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetNGFR
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-NGFR Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4804
  • Target name
    NGFR
  • Target description
    nerve growth factor receptor
  • Target synonyms
    CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR, tumor necrosis factor receptor superfamily member 16, NGF receptor, TNFR superfamily, member 16, low affinity neurotrophin receptor p75NTR, low-affinity nerve growth factor receptor, low-affinity nerve growth factor receptor p75NGFR, low-affinity nerve growth factor receptor p75NGR, p75 ICD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08138
  • Protein Name
    Tumor necrosis factor receptor superfamily member 16
  • Scientific Description
    NGFR Polyclonal Antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of p75 NGF Receptor/NGFR using anti-p75 NGF Receptor/NGFR antibody (A01187). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p75 NGF Receptor/NGFR antigen affinity purified polyclonal antibody (Catalog # A01187) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p75 NGF Receptor/NGFR at approximately 45KD,75KD. The expected band size for p75 NGF Receptor/NGFR is at 45KD,75KD.
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