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Western blot analysis using Anti-NLRP2 antibody HPA020765 (A) shows similar pattern to independent antibody HPA021183 (B).
Western blot analysis using Anti-NLRP2 antibody HPA020765 (A) shows similar pattern to independent antibody HPA021183 (B).
Western blot analysis using Anti-NLRP2 antibody HPA020765 (A) shows similar pattern to independent antibody HPA021183 (B).

Anti-NLRP2-25ul

HPA020765
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NLRP2-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NX02
  • Protein Name
    NACHT, LRR and PYD domains-containing protein 2
  • Scientific Description
    Rabbit Polyclonal Anti-NLRP2 Antibody against Human NLR family, pyrin domain containing 2. Validated for Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

Datasheet

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MSDS

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Figure 1. Western blot analysis of NLRP2 using anti-NLRP2 antibody (A04819-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP2 antigen affinity purified polyclonal antibody (Catalog # A04819-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP2 at approximately 121KD. The expected band size for NLRP2 is at 121KD.
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