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Immunofluorescent staining of human cell line A549 shows localization to cytosol, the Golgi apparatus & vesicles.
Immunofluorescent staining of human cell line A549 shows localization to cytosol, the Golgi apparatus & vesicles.
Immunofluorescent staining of human cell line A549 shows localization to cytosol, the Golgi apparatus & vesicles.

Anti-NLRP2 Antibody

HPA021183
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNLRP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NLRP2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55655
  • Target name
    NLRP2
  • Target description
    NLR family pyrin domain containing 2
  • Target synonyms
    CLR19.9, NALP2, NBS1, OZEMA18, PAN1, PYPAF2, NACHT, LRR and PYD domains-containing protein 2, NACHT, leucine rich repeat and PYD containing 2, PYRIN domain and NACHT domain-containing protein 1, PYRIN-containing APAF1-like protein 2, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 2, nucleotide-binding site protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NX02
  • Protein Name
    NACHT, LRR and PYD domains-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NLRP2 using anti-NLRP2 antibody (A04819-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP2 antigen affinity purified polyclonal antibody (Catalog # A04819-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP2 at approximately 121KD. The expected band size for NLRP2 is at 121KD.
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