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Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.

Anti-NLRP7 Antibody

HPA051382
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNLRP7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NLRP7 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID199713
  • Target name
    NLRP7
  • Target description
    NLR family pyrin domain containing 7
  • Target synonyms
    CLR19.4, HYDM, NALP7, NOD12, PAN7, PYPAF3, NACHT, LRR and PYD domains-containing protein 7, NACHT, LRR and PYD containing protein 7, NACHT, leucine rich repeat and PYD containing 7, PYRIN-containing Apaf1-like protein 3, nucleotide-binding oligomerization domain protein 12, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WX94
  • Protein Name
    NACHT, LRR and PYD domains-containing protein 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NLRP7 using anti-NLRP7 antibody (A07838-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP7 antigen affinity purified polyclonal antibody (Catalog # A07838-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP7 at approximately 112 kDa. The expected band size for NLRP7 is at 112 kDa.
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