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Goat anti-NLRP7

EB12896
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetNLRP7
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NLRP7 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID199713
  • Target name
    NLRP7
  • Target description
    NLR family pyrin domain containing 7
  • Target synonyms
    CLR19.4; HYDM; NACHT, leucine rich repeat and PYD containing 7; NACHT, LRR and PYD containing protein 7; NACHT, LRR and PYD domains-containing protein 7; NALP7; NOD12; nucleotide-binding oligomerization domain protein 12; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 7; PAN7; PYPAF3; PYRIN-containing Apaf1-like protein 3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_631915.2; NP_996611.2; NP_001120727.1. Purification: Antigen affinity purified. Names and symbols: NLRP7; NLR family, pyrin domain containing 7; HYDM; PAN7; NALP7; NOD12; PYPAF3; CLR19.4
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NLRP7 using anti-NLRP7 antibody (A07838-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP7 antigen affinity purified polyclonal antibody (Catalog # A07838-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP7 at approximately 112 kDa. The expected band size for NLRP7 is at 112 kDa.
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