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Immunohistochemical staining of human ovary shows nuclear positivity in ovarian stroma cells.
Immunohistochemical staining of human ovary shows nuclear positivity in ovarian stroma cells.
Immunohistochemical staining of human ovary shows nuclear positivity in ovarian stroma cells.

Anti-NOB1 Antibody

HPA049430
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNOB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NOB1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID28987
  • Target name
    NOB1
  • Target description
    NIN1 (RPN12) binding protein 1 homolog
  • Target synonyms
    ART-4, MST158, MSTP158, NOB1P, PSMD8BP1, RNA-binding protein NOB1, NIN1/PSMD8 binding protein 1 homolog, PSMD8 binding protein 1, adenocarcinoma antigen recognized by T lymphocytes 4, nin one binding protein, phosphorylation regulatory protein HP-10, protein ART-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULX3
  • Protein Name
    RNA-binding protein NOB1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of NOB1 using anti-NOB1 antibody (A03504-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOB1 antigen affinity purified polyclonal antibody (Catalog # A03504-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOB1 at approximately 47 kDa. The expected band size for NOB1 is at 47 kDa.
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