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Western Blot Positive WB detected in: Hela whole cell lysate, Mouse brain tissue, Mouse kidney tissue, Mouse stomach tissue All lanes: NOB1 antibody at 1.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Mouse brain tissue, Mouse kidney tissue, Mouse stomach tissue All lanes: NOB1 antibody at 1.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Mouse brain tissue, Mouse kidney tissue, Mouse stomach tissue All lanes: NOB1 antibody at 1.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa

NOB1 Antibody

CSB-PA892472LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNOB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NOB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID28987
  • Target name
    NOB1
  • Target description
    NIN1 (RPN12) binding protein 1 homolog
  • Target synonyms
    ART-4, MST158, MSTP158, NOB1P, PSMD8BP1, RNA-binding protein NOB1, NIN1/PSMD8 binding protein 1 homolog, PSMD8 binding protein 1, adenocarcinoma antigen recognized by T lymphocytes 4, nin one binding protein, phosphorylation regulatory protein HP-10, protein ART-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULX3
  • Protein Name
    RNA-binding protein NOB1
  • Scientific Description
    May play a role in mRNA degradation.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOB1 using anti-NOB1 antibody (A03504-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOB1 antigen affinity purified polyclonal antibody (Catalog # A03504-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOB1 at approximately 47 kDa. The expected band size for NOB1 is at 47 kDa.
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