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Immunohistochemical staining of human caudate shows strong positivity in neurons.
Immunohistochemical staining of human caudate shows strong positivity in neurons.
Immunohistochemical staining of human caudate shows strong positivity in neurons.

Anti-NOS1 Antibody

HPA069509
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNOS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NOS1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4842
  • Target name
    NOS1
  • Target description
    nitric oxide synthase 1
  • Target synonyms
    IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS, nitric oxide synthase 1, NOS type I, constitutive NOS, neuronal NOS, nitric oxide synthase 1 (neuronal), peptidyl-cysteine S-nitrosylase NOS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29475
  • Protein Name
    Nitric oxide synthase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of nNOS (neuronal)/NOS1 using anti-nNOS (neuronal)/NOS1 antibody (A01070-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-nNOS (neuronal)/NOS1 antigen affinity purified polyclonal antibody (Catalog # A01070-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for nNOS (neuronal)/NOS1 at approximately 161 kDa. The expected band size for nNOS (neuronal)/NOS1 is at 161 kDa.
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