More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Notch1 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, DAB staining.

Notch1 Polyclonal Antibody

BS-1335R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNOTCH1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Notch1/MOTC Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4851
  • Target name
    NOTCH1
  • Target description
    notch receptor 1
  • Target synonyms
    AOS5; AOVD1; hN1; neurogenic locus notch homolog protein 1; notch 1; Notch homolog 1, translocation-associated; TAN1; translocation-associated notch protein TAN-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46531
  • Protein Name
    Neurogenic locus notch homolog protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Sun Z et al., 2020 Jul, Cell Mol Neurobiol
    Read more
  • In Vivo Study on the Effects of Xiaoaiping on the Stemness of Hepatocellular Carcinoma Cells. Zhan J et al., 2019, Evid Based Complement Alternat Med
    Read more
  • The Notch signaling pathway inhibitor Dapt alleviates autism-like behavior, autophagy and dendritic spine density abnormalities in a valproic acid-induced animal model of autism. Zhang Y et al., 2019 Aug 30, Prog Neuropsychopharmacol Biol Psychiatry
    Read more
  • A photothermal-triggered nitric oxide nanogenerator combined with siRNA for precise therapy of osteoarthritis by suppressing macrophage inflammation. Chen X et al., 2019 Apr 4, Nanoscale
    Read more
  • Association of Notch-1, osteopontin and stem-like cells in ENU-glioma malignant process. Bulnes S et al., 2018 Jul 31, Oncotarget
    Read more
  • Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway. Zou Q et al., 2018 May, EBioMedicine
    Read more

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-NOTCH1 Antibody144-07636
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetNOTCH1
  • SizePrice
Product group Antibodies
Biomatik
Translocation Associated Notch Homolog 1 (TAN1) Polyclonal AntibodyCAU30159
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetNOTCH1
  • SizePrice
Product group Antibodies
Assay Genie
Notch1 Monoclonal AntibodyCAB19090
TargetNOTCH1
  • SizePrice
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA015949LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
NOTCH1 AntibodyCSB-PA015949LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetNOTCH1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
NOTCH1 AntibodyLS-C402721
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetNOTCH1
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Notch 1 [E6]Ab00175-23.0
ApplicationsELISA, ImmunoHistoChemistry
TargetNOTCH1
  • SizePrice
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
Anti-NOTCH1 AntibodyHPA067168
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetNOTCH1
  • SizePrice
Anti-Notch1 Antibody
Product group Antibodies
Antibodies.com
Anti-Notch1 AntibodyA286055
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-Notch1 Antibody, Affinity PurifiedA301-894A
ApplicationsImmunoPrecipitation
TargetNOTCH1
  • SizePrice
Figure 1. Western blot analysis of Notch1 using anti-Notch1 antibody (A00033-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Notch1 antigen affinity purified polyclonal antibody (Catalog # A00033-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Notch1 at approximately 272KD. The expected band size for Notch1 is at 272KD.
Product group Antibodies
Boster Bio
Anti-Notch1 Antibody Picoband(r)A00033-2-CARRIER-FREE
ApplicationsWestern Blot
TargetNOTCH1
  • SizePrice