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Immunohistochemical staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.

Anti-NPC1 Antibody

HPA026618
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetNPC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NPC1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4864
  • Target name
    NPC1
  • Target description
    NPC intracellular cholesterol transporter 1
  • Target synonyms
    Niemann-Pick C1 protein; NPC; NPC intracellular cholesterol transporter 1; POGZ; SLC65A1; truncated Niemann-Pick C1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15118
  • Protein Name
    NPC intracellular cholesterol transporter 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Niemann Pick C1 using anti-Niemann Pick C1 antibody (A00428-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Niemann Pick C1 antigen affinity purified polyclonal antibody (Catalog # A00428-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Niemann Pick C1 at approximately 170KD. The expected band size for Niemann Pick C1 is at 142KD.
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