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Niemann Pick C1 Antibody (YA2290, Rabbit)

HY-P82545
MedChem Express
TargetNPC1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Niemann Pick C1 Antibody (YA2290, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4864
  • Target name
    NPC1
  • Target description
    NPC intracellular cholesterol transporter 1
  • Target synonyms
    NPC, POGZ, SLC65A1, NPC intracellular cholesterol transporter 1, Niemann-Pick C1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Niemann Pick C1 Antibody (YA2290) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Niemann Pick C1.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Niemann Pick C1 using anti-Niemann Pick C1 antibody (A00428-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Niemann Pick C1 antigen affinity purified polyclonal antibody (Catalog # A00428-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Niemann Pick C1 at approximately 170KD. The expected band size for Niemann Pick C1 is at 142KD.
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