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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-NR2C2 Antibody

HPA006313
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNR2C2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NR2C2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7182
  • Target name
    NR2C2
  • Target description
    nuclear receptor subfamily 2 group C member 2
  • Target synonyms
    TAK1, TR4, nuclear receptor subfamily 2 group C member 2, Nuclear hormone receptor TR4, orphan nuclear receptor TAK1, orphan nuclear receptor TR4, orphan receptor TR4, testicular nuclear receptor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49116
  • Protein Name
    Nuclear receptor subfamily 2 group C member 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NR2C2 using anti-NR2C2 antibody (A02752-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human HEL whole cell lysates, Lane 7: human U20S whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse thymus tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR2C2 antigen affinity purified polyclonal antibody (Catalog # A02752-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR2C2 at approximately 68 kDa. The expected band size for NR2C2 is at 68 kDa.
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