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Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.

Anti-NR3C2 Antibody

HPA074979
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNR3C2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NR3C2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4306
  • Target name
    NR3C2
  • Target description
    nuclear receptor subfamily 3 group C member 2
  • Target synonyms
    aldosterone receptor; MCR; mineralocorticoid receptor; mineralocorticoid receptor 1; mineralocorticoid receptor 2; mineralocorticoid receptor delta; MLR; MR; NR3C2VIT; nuclear receptor subfamily 3, group C, member 2 variant 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08235
  • Protein Name
    Mineralocorticoid receptor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC-P analysis of human colon carcinoma tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Mineralocorticoid receptor Polyclonal Antibody, Unconjugated (bs-1850R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.
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Figure 2. IF analysis of NR3C2 using anti-NR3C2 antibody (PB9765) and anti-Tubulin Alpha antibody (M03989-3). NR3C2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 microg/mL rabbit anti-NR3C2 Antibody (PB9765) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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