IHC-P analysis of human colon carcinoma tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
![IHC-P analysis of human kidney tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22774/GTX22774_1100_IHC-P_w_23060620_601.webp "IHC-P analysis of human kidney tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22774/GTX22774_1101_IHC-P_w_23060620_472.webp "IHC-P analysis of human tonsil tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
![ICC/IF analysis of HEK293 cells using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22774/GTX22774_426_ICC-IF_w_23060620_642.webp "ICC/IF analysis of HEK293 cells using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22774/GTX22774_427_ICC-IF_w_23060620_861.webp "ICC/IF analysis of HeLa cells using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of HepG2 cells using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22774/GTX22774_428_ICC-IF_w_23060620_819.webp "ICC/IF analysis of HepG2 cells using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human colon carcinoma tissue using GTX22774 Mineralocorticoid Receptor antibody [H10E4C9F]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
Mineralocorticoid Receptor antibody [H10E4C9F]
GTX22774
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityChicken, Human, Mouse, Rabbit, Rat, Sheep
TargetNR3C2
Overview
- SupplierGeneTex
- Product NameMineralocorticoid Receptor antibody [H10E4C9F]
- Delivery Days Customer9
- Antibody SpecificityThe antibody can also react with the steroid binding domain for aldosterone on other receptors.
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:100-1:200. IHC-P: 1:100. FACS: 1/20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDH10E4C9F
- Concentration1.4 mg/ml
- ConjugateUnconjugated
- Gene ID4306
- Target nameNR3C2
- Target descriptionnuclear receptor subfamily 3 group C member 2
- Target synonymsaldosterone receptor; MCR; mineralocorticoid receptor; mineralocorticoid receptor 1; mineralocorticoid receptor 2; mineralocorticoid receptor delta; MLR; MR; NR3C2VIT; nuclear receptor subfamily 3, group C, member 2 variant 3
- HostMouse
- IsotypeIgG1
- Protein IDP08235
- Protein NameMineralocorticoid receptor
- Scientific DescriptionThis gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
- ReactivityChicken, Human, Mouse, Rabbit, Rat, Sheep
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203