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Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Anti-NRF1 Antibody

HPA029329
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Chip-exoSeq
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNRF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NRF1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Chip-exoSeq
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4899
  • Target name
    NRF1
  • Target description
    nuclear respiratory factor 1
  • Target synonyms
    alpha palindromic-binding protein; ALPHA-PAL; nuclear respiratory factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16656
  • Protein Name
    Nuclear respiratory factor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: Nuclear respiratory factor 1 antibody at 3ug/ml Lane 1: Mouse skeletal muscle tissue Lane 2: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 47, 37, 56 kDa Observed band size: 54 kDa
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Mouse heart and muscle lysates probed with Rabbit Anti-NRF-1 Polyclonal Antibody, Unconjugated (bs-1342R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
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Figure 2. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). NRF1 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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