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Western blot All lanes: Nuclear respiratory factor 1 antibody at 3ug/ml Lane 1: Mouse skeletal muscle tissue Lane 2: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 47, 37, 56 kDa Observed band size: 54 kDa
Western blot All lanes: Nuclear respiratory factor 1 antibody at 3ug/ml Lane 1: Mouse skeletal muscle tissue Lane 2: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 47, 37, 56 kDa Observed band size: 54 kDa
Western blot All lanes: Nuclear respiratory factor 1 antibody at 3ug/ml Lane 1: Mouse skeletal muscle tissue Lane 2: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 47, 37, 56 kDa Observed band size: 54 kDa

NRF1 Antibody

CSB-PA016076ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNRF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NRF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4899
  • Target name
    NRF1
  • Target description
    nuclear respiratory factor 1
  • Target synonyms
    ALPHA-PAL, nuclear respiratory factor 1, alpha palindromic-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16656
  • Protein Name
    Nuclear respiratory factor 1
  • Scientific Description
    Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 2. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). NRF1 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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